產品說明:

The most popular E. coli strain for everyday cloning applications.

It supports blue/white screening for easy selection of recombinant DNA with X-Gal.

Carries recA1 mutation that eliminates homologous recombination ensuring insert stability.

Carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep.

Useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

 

Genotype

F¯ endA1 hsdR17(rk¯, mk¯) supE44 thi-1 λ¯recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 Φ80d lacZ ΔM15 deoR

 

 

特色:

  • Suitable for cloning with large plasmid and cDNA library construction
  • Allows blue-white colony screening.

 

應用:

  • Cloning and subcloning
  • Scale-up application
  • Blue/white screening

SMB DH5α Competent Cells [DH5α]

  • 貨號 品名 建議用途 包裝
    SMBE607 SMB Economic DH5a, >1 x 108 cfu/ug
    • Subcloning
    • General cloning

    100 μL/vial, 80 vials

    Control Plasmid (pUC19): 5 μL (10-4 μg/μL)
    SMBE678 SMB Flagship DH5a, >5 x 108 cfu/ug
    • High efficiency cloning
    • Cloning low abundance transcripts
    • General cloning
    • Gene library

    100 μL/vial, 80 vials

    Control Plasmid (pUC19): 5 μL (10-4 μg/μL)
    SMBE609 SMB DH5a, >109 cfu/ug
    • Super high efficiency cloning
    • Cloning lowest abundance transcripts
    • Gene library
    50 μl/well, 96 wells

     

     

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