![SMB DH5α Competent Cells [DH5α]](https://static.wixstatic.com/media/8e1ad8_05dfdd423093445d9367912618271d5e~mv2.png/v1/fill/w_498,h_498,al_c,lg_1/8e1ad8_05dfdd423093445d9367912618271d5e~mv2.png)
產品說明:
The most popular E. coli strain for everyday cloning applications.
It supports blue/white screening for easy selection of recombinant DNA with X-Gal.
Carries recA1 mutation that eliminates homologous recombination ensuring insert stability.
Carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep.
Useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
Genotype
F¯ endA1 hsdR17(rk¯, mk¯) supE44 thi-1 λ¯recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 Φ80d lacZ ΔM15 deoR
特色:
- Suitable for cloning with large plasmid and cDNA library construction
- Allows blue-white colony screening.
應用:
- Cloning and subcloning
- Scale-up application
- Blue/white screening
SMB DH5α Competent Cells [DH5α]
貨號 品名 建議用途 包裝 SMBE607 SMB Economic DH5a, >1 x 108 cfu/ug - Subcloning
- General cloning
100 μL/vial, 80 vials
Control Plasmid (pUC19): 5 μL (10-4 μg/μL)SMBE678 SMB Flagship DH5a, >5 x 108 cfu/ug - High efficiency cloning
- Cloning low abundance transcripts
- General cloning
- Gene library
100 μL/vial, 80 vials
Control Plasmid (pUC19): 5 μL (10-4 μg/μL)SMBE609 SMB DH5a, >109 cfu/ug - Super high efficiency cloning
- Cloning lowest abundance transcripts
- Gene library
50 μl/well, 96 wells