The most popular E. coli strain for everyday cloning applications.
It supports blue/white screening for easy selection of recombinant DNA with X-Gal.
Carries recA1 mutation that eliminates homologous recombination ensuring insert stability.
Carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep.
Useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).
F¯ endA1 hsdR17(rk¯, mk¯) supE44 thi-1 λ¯recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 Φ80d lacZ ΔM15 deoR
- Suitable for cloning with large plasmid and cDNA library construction
- Allows blue-white colony screening.
- Cloning and subcloning
- Scale-up application
- Blue/white screening