產品說明:

High levels of protein expression

Carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction

The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability.

The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.

 

Genotype

F¯ hsdS gal (λ cIts857 ind1 Sam7 nin5 lacUV5-T7 gene l)

 

 

特色:

  • Lack of Lon and Omp proteases allows high-level protein expression and accumulation
  • Easy gene induction

 

 

應用:

  • IPTG induced gene expression in E. coli
  • Ideal for high level expression of non-toxic recombinant protein expression using T7-based expression vectors
  • Subcloning

SMB BL21 Competent Cells [BL21(DE3)]