High levels of protein expression
Carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction
The mutated rne gene (rne131) encodes a truncated RNase E enzyme that lacks the ability to degrade mRNA, resulting in an increase in mRNA stability.
The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired.
F¯ hsdS gal (λ cIts857 ind1 Sam7 nin5 lacUV5-T7 gene l)
- Lack of Lon and Omp proteases allows high-level protein expression and accumulation
- Easy gene induction
- IPTG induced gene expression in E. coli
- Ideal for high level expression of non-toxic recombinant protein expression using T7-based expression vectors