SMB JM109 Competent Cells [JM109]

介紹

A a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA.

Carries the F’ episome which allows blue/white screening for recombinant DNA.

A popular strain for routine subcloning.

Utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously.

Sensitive to all common antibiotics.

This product can become a powerful cloning tool when it is used in combination with ligase.

Grows fast, colonies to show up within 8 hours. Try this strain when you are in urgent.

Genotype

F' traD36 proA +  proB +  lacIq Δ(lacZ)M15 Δ(lac-proAB) supE44 hsdR17 recA1 gyrA96 thi¯1 endA1 relA1 e14¯ λ¯
 

應用:

• Preparation of ssDNA
• Construction of gene library
• Subcloning