Real-Time PCR (qPCR) Premix

SMB Real-Time PCR Premix

SMB試劑
特點
Specificity: Hotstart DNA polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Sensitivity: Detects low copy number targets
Wide linear range: Accurate quantification across 9 orders of magnitude
Reproducibility and convenience: Ready-to-use 2× master mix minimizes pipetting error and reduces set-up time

介紹

A ready-to-use, 2× concentrated PCR premix with Hotstart DNA polymerase, fluorescent dye (SYBR Green I format only), optimized reaction buffer and dNTPs.

With/without ROX is optional.

Suitable for classical 3-steps PCR protocol

8 different packages: see the order information

SYBR Green I format (# SMBT103 or # SMBT104)

Suitable for real-time PCR and semi qRT-PCR.

High sensitivity, wide dynamic range and reproducibility for quantification.

Taq Man format (# SMBT105 or # SMBT106)

Premix contains all reagents (except for primers, probe and template).

For real-time PCR

Performed by addition of various probes.

Provides good specificity and amplification efficiency

Molecular Beacon is also compatible.
 

應用:

  • Real-time PCR
  • Semi qRT-PCR
  • Quick and accurate detection and quantification of target gene through real-time PCR

規格與訂購資訊

型號
內容物 包裝
SMBT103-100P
SMB Real-Time PCR Premix(2X, SYBR Green) 1.25 mL (100 reactions)
SMBT103- 400P
SMB Real-Time PCR Premix(2X, SYBR Green) 4 × 1.25 mL (400 reactions)
SMBT104-100P
SMB Real-Time PCR Premix(2X, SYBR Green, ROX) 1.25 mL (100 reactions)
SMBT104-400P
SMB Real-Time PCR Premix(2X, SYBR Green, ROX) 4 × 1.25 mL (400 reactions)
SMBT105-100P
SMB Real-Time PCR Premix(2X, TaqMan) 1.25 mL (100 reactions)
SMBT105-400P
SMB Real-Time PCR Premix(2X, TaqMan) 4 × 1.25 mL (400 reactions)
SMBT106-100P
SMB Real-Time PCR Premix(2X, TaqMan, ROX) 1.25 mL (100 reactions)
SMBT106-400P
SMB Real-Time PCR Premix(2X, TaqMan, ROX) 4 × 1.25 mL (400 reactions)
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