PCR Premix 與 DNA 聚合酶
SMB HotStart DNA Polymerase & PremixSMB試劑
- Yield: 1 kb/min
- Error rate: 1×10-5 / nucleotide / cycle
- Amplicons: up to 600 bps
- Able to amplify single gene copy
A chemical-mediated highly sensitive HotStart DNA polymerase
Activated after heating, greatly reduces non-specific amplification at the room temperature
Premix version is simple & time-saving, less contamination and convenient.
2× concentrated ready to use premix (including HotStart DNA polymerase, dNTPs, optimized buffers, and loading dye (optional)).
The only step of premix version is to add DNA template and primers into the reaction mix.
5 different packages: see the order information
- High throughput hot-start PCR
- Highly specific amplification of complex genomic and cDNA templates
- Amplification of low copy DNA targets
- Generation of PCR products for TA cloning
- Diagnostic analysis
SMB Hotstart DNA Polymerase (w/ dNTPs)
|100 μL (5 U/μl)*|
|SMB Hotstart DNA Polymerase (w/o dNTPs)||100 μL (5 U/μl)*|
|SMB Hotstart DNA Polymerase Premix w/o dye||2 × 1.25 mL (100 reactions)|
|SMB Hotstart DNA Polymerase Premix w/ dye||2 × 1.25 mL (100 reactions)|
|SMB Hotstart DNA Polymerase Premix w/o dye||4 × 1.25 mL (200 reactions)|
*Unit Definition: The amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72℃.