A ready-to-use, 2× concentrated PCR premix with Hotstart DNA polymerase, fluorescent dye (SYBR Green I format only), optimized reaction buffer and dNTPs.
With/without ROX is optional.
Suitable for classical 3-steps PCR protocol
8 different packages: see the order information
SYBR Green I format (# SMBT103 or # SMBT104)
Suitable for real-time PCR and semi qRT-PCR.
High sensitivity, wide dynamic range and reproducibility for quantification.
Taq Man format (# SMBT105 or # SMBT106)
Premix contains all reagents (except for primers, probe and template).
For real-time PCR
Performed by addition of various probes.
Provides good specificity and amplification efficiency
Molecular Beacon is also compatible.
Hotstart DNA polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
Detects low copy number targets
- Wide linear range
Accurate quantification across 9 orders of magnitude
- Reproducibility and convenience
Ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
- Real-time PCR
- Semi qRT-PCR
- Quick and accurate detection and quantification of target gene through real-time PCR
SMB Real-Time PCR Premix
貨號 品名 包裝 SMBT103-100P SMB Real-Time PCR Premix(2X, SYBR Green) 1.25 mL (100 reactions) SMBT103- 400P SMB Real-Time PCR Premix(2X, SYBR Green) 4 × 1.25 mL (400 react