產品說明:
A ready-to-use, 2× concentrated PCR premix with Hotstart DNA polymerase, fluorescent dye (SYBR Green I format only), optimized reaction buffer and dNTPs.
With/without ROX is optional.
Suitable for classical 3-steps PCR protocol
8 different packages: see the order information
SYBR Green I format (# SMBT103 or # SMBT104)
Suitable for real-time PCR and semi qRT-PCR. 
High sensitivity, wide dynamic range and reproducibility for quantification.
Taq Man format (# SMBT105 or # SMBT106)
Premix contains all reagents (except for primers, probe and template).
For real-time PCR
Performed by addition of various probes.
Provides good specificity and amplification efficiency
Molecular Beacon is also compatible.
 
 
特色:
	
Specificity
Hotstart DNA polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
	
Sensitivity
Detects low copy number targets
	
Wide linear range
Accurate quantification across 9 orders of magnitude
	
Reproducibility and convenience
Ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
 
 
應用:
	
Real-time PCR
	
Semi qRT-PCR
	
Quick and accurate detection and quantification of target gene through real-time PCR