產品說明:

  • Point mutations of MMLV RTase by genetically engineering
  • Longer half-life, less RNase H activity and greater thermal stability than MMLV RTase.
  • Reduced RNase H activity for more full-length cDNA
  • Higher specificity and more cDNA yields.
  • Ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)+ RNA samples.
  • Synthesizes a complementary DNA strand from total RNA, mRNA, or an RNA : DNA hybrid.
  • 3 different package and RNase inhibitor: see the order information

 

特色:

  • Half-life: 100 minutes at 50°C for the highest cDNA yields
  • cDNA lengh: > 6kb
  • Full activity at 50°C for increased specificity with GSP
  • Ability to increase RT units without inhibiting subsequent PCR

 

應用:

  • Synthesis of first-strand cDNA
  • Array labeling
  • cDNA libraries
  • RT-PCR, primer extension, and 3' and 5' RACE

SMB Hipoint Reverse Transcriptase

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