SMB DH5α Competent Cells [DH5α]

SMB試劑

特點: Suitable for cloning with large plasmid and cDNA library construction; Allows blue-white colony screening.

The most popular E. coli strain for everyday cloning applications.

It supports blue/white screening for easy selection of recombinant DNA with X-Gal.

Carries recA1 mutation that eliminates homologous recombination ensuring insert stability.

Carries endA1 mutations that greatly improve the quality of plasmid DNA and yield prepared from mini-prep.

Useful for the transformation of large plasmids and two-hybrid systems (up to 40 kb).

Genotype

F¯ endA1 hsdR17(rk¯, mk¯) supE44 thi-1 λ¯recA1 gyrA96 relA1 Δ(argF-lacZYA) U169 Φ80d lacZ ΔM15 deoR

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規格與訂購資訊

貨號	內容物	建議用途	包裝
SMBE607	SMB Economic DH5a, >1 x 108 cfu/ug	Subcloning General cloning	100 μL/vial, 80 vials Control Plasmid (pUC19): 5 μL (10-4 μg/μL)
SMBE678	SMB Flagship DH5a, >5 x 108 cfu/ug	High efficiency cloning Cloning low abundance transcripts General cloning Gene library	100 μL/vial, 80 vials Control Plasmid (pUC19): 5 μL (10-4 μg/μL)
SMBE609	SMB DH5a, >109 cfu/ug	Super high efficiency cloning Cloning lowest abundance transcripts Gene library	50 μl/well, 96 wells

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