SMB Real-time RT-PCR Kit

介紹

SMB Real-time SYBR Green RT-PCR system provides user with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SYBR Green. SMB Hipoint RT, HotStart DNA polymerase and all the components for Real-time SYBR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer-dimers.

TaqMan detection system uses a designed fluorogenic probe to detect target PCR products during real-time PCR cycle. Fluorescent is detected when the quencher of probe is removed during the PCR extension cycle. SMB Real-time TaqMan RT-PCR kit complete reverse transcription and TaqMan Real-time PCR in a single tube. SMB Hipoint RT, HotStart DNA polymerase and all the components necessary for performing TaqMan Real-time PCR from RNA template are specially prepared to ensure high specificity and high sensitivity.
 

應用:

• Validation of siRNA-mediated gene knockdown 
• Detection of multiple gene expressions