PCR Premix 與 DNA 聚合酶

SMB HiFi DNA Polymerase & Premix

Yield: 1 kb/5secs (see the figure)
Error rate: 8.3 × 10-6 / nucleotide / cycle
Generates 3’-dA overhangs
Amplicons: up to 6kb
Robust amplification with minimal optimization


Ideal DNA polymerase for amplification of DNA fragments that require high-fidelity.

An optimal blend of SMB Taq DNA polymerase and Accu DNA polymerase, take into consideration both of yield and proofreading.

dUTP, dITP and primers containing these nucleotides inhibit Accu DNA polymerase activity. Not recommended to use in PCR reaction with HiFi.

Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).

2 different packages: see the order information

The other detail, please see the “SMB Taq DNA polymerase” and “SMB Accu DNA polymerase”.


  • Cloning and expression
  • Blunt end amplification products
  • Mutation analysis
  • Efficient & highly sensitive PCR use
  • Routine PCR amplification of DNA fragments up to 6 kb from genomic DNA
  • RT-PCR


內容物 包裝
SMB HiFi DNA Polymerase (w/ dNTPs) 100 μL (5 U/μl)*
SMB HiFi DNA Polymerase (w/o dNTPs) 100 μL (5 U/μl)*

*Unit Definition: The amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72℃.