PCR Premix 與 DNA 聚合酶

SMB Accu DNA Polymerase

Yield: 1 kb/2min
Highly thermostable: remains 95% activity after 2 hours incubation at 95℃
Eight times more accurate than Taq DNA polymerase
Error rate: 2.6 × 10-6 / nucleotide / cycle
Generates blunt-end PCR products
Amplicons: up to 3kb


DNA polymerase derived from Pyrococcus Sp..

Catalyzes the incorporation of nucleotides into dsDNA in the 5'→3' direction in the presence of Mg2+ at 70 ~ 80℃.

Exhibits 3'→5' exonuclease (proofreading) activity, but has no detectable 5'→3' exonuclease activity.

Exhibits deoxynucleotidyl transferase activity, results in the addition of extra adenines at the 3’-end of PCR products, required for TA cloning

Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).

dUTP and dITP inhibit Accu DNA polymerase activity. Not recommended to use in PCR reaction with Accu.

3 different packages (including ready to use premix version, convenient and economic): see the order information

Produced by E. coli


  • Cloning
  • Amplification
  • Mutation analysis
  • Accurate polymerization
  • Routine PCR amplification of DNA fragments up to 3 kb
  • RT-PCR
  • Blunt end amplification products


內容物 包裝
SMB Accu DNA Polymerase (w/ dNTPs) 100 μL (5 U/μl)*
SMB Accu DNA Polymerase (w/o dNTPs) 100 μL (5 U/μl)*
Accu DNA Polymerase Premix (2×) with Green dye 2 × 1.25 mL (100 reactions)

*Unit Definition: The amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72℃.