A novel Hotstart DNA polymerase, capable of catalyzing DNA amplification in a fast 2-step PCR protocol.
Greatly shortens the running time of real-time quantitative PCR by around 1 hour compared to traditional protocol
In addition to high sensitivity, wide dynamic range, and good reproducibility, this special formula precisely meets current researchers’ needs for performing gene detection (qPCR) and quantification of gene expression (semi qRT-PCR) in a high speed and/or high-throughput manner.
A ready-to-use, 2× concentrated PCR premix with Hotstart DNA polymerase, fluorescent dye (SYBR Green I format only), optimized reaction buffer and dNTPs.
With/without ROX is optional.
8 different packages: see the order information
SYBR Green I format (# SMBT107 or # SMBT108)
Suitable for real-time PCR and semi qRT-PCR.
High sensitivity, wide dynamic range and reproducibility for quantification.
Taq Man format (# SMBT109 or # SMBT110)
Premix contains all reagents (except for primers, probe and template).
For real-time PCR
Performed by addition of various probes.
Provides good specificity and amplification efficiency
Molecular Beacon is also compatible.
Shortens the running time of real-time quantitative PCR by around 1 hour
Hotstart DNA polymerase and the optimized buffer eliminates non-specific amplification and formation of primer dimers
Detects low copy number targets
- Wide linear range
Accurate quantification across 9 orders of magnitude
- Reproducibility and convenience
Ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
- Real-time PCR
- Semi qRT-PCR
- Quick and accurate detection and quantification of target gene through real-time PCR