產品說明:

A novel Hotstart DNA polymerase, capable of catalyzing DNA amplification in a fast 2-step PCR protocol.

Greatly shortens the running time of real-time quantitative PCR by around 1 hour compared to traditional protocol

In addition to high sensitivity, wide dynamic range, and good reproducibility, this special formula precisely meets current researchers’ needs for performing gene detection (qPCR) and quantification of gene expression (semi qRT-PCR) in a high speed and/or high-throughput manner.

A ready-to-use, 2× concentrated PCR premix with Hotstart DNA polymerase, fluorescent dye (SYBR Green I format only), optimized reaction buffer and dNTPs.

With/without ROX is optional.

8 different packages: see the order information

SYBR Green I format (# SMBT107 or # SMBT108)

Suitable for real-time PCR and semi qRT-PCR.

High sensitivity, wide dynamic range and reproducibility for quantification.

Taq Man format (# SMBT109 or # SMBT110)

Premix contains all reagents (except for primers, probe and template).

For real-time PCR

Performed by addition of various probes.

Provides good specificity and amplification efficiency

Molecular Beacon is also compatible.

 

 

特色:

  • Fast

Shortens the running time of real-time quantitative PCR by around 1 hour

  • Specificity

Hotstart DNA polymerase and the optimized buffer eliminates non-specific amplification and formation of primer dimers

  • Sensitivity

Detects low copy number targets

  • Wide linear range

Accurate quantification across 9 orders of magnitude

  • Reproducibility and convenience