產品說明:
A novel Hotstart DNA polymerase, capable of catalyzing DNA amplification in a fast 2-step PCR protocol.
Greatly shortens the running time of real-time quantitative PCR by around 1 hour compared to traditional protocol
In addition to high sensitivity, wide dynamic range, and good reproducibility, this special formula precisely meets current researchers’ needs for performing gene detection (qPCR) and quantification of gene expression (semi qRT-PCR) in a high speed and/or high-throughput manner.
A ready-to-use, 2× concentrated PCR premix with Hotstart DNA polymerase, fluorescent dye (SYBR Green I format only), optimized reaction buffer and dNTPs.
With/without ROX is optional.
8 different packages: see the order information
SYBR Green I format (# SMBT107 or # SMBT108)
Suitable for real-time PCR and semi qRT-PCR. 
High sensitivity, wide dynamic range and reproducibility for quantification.
Taq Man format (# SMBT109 or # SMBT110)
Premix contains all reagents (except for primers, probe and template).
For real-time PCR
Performed by addition of various probes.
Provides good specificity and amplification efficiency
Molecular Beacon is also compatible.
 
 
特色:
	
Fast
Shortens the running time of real-time quantitative PCR by around 1 hour 
	
Specificity 
Hotstart DNA polymerase and the optimized buffer eliminates non-specific amplification and formation of primer dimers 
	
Sensitivity
Detects low copy number targets 
	
Wide linear range
Accurate quantification across 9 orders of magnitude 
	
Reproducibility and convenience 
Ready-to-use 2× master mix minimizes pipetting error and reduces set-up time
 
應用:
	
Real-time PCR
	
Semi qRT-PCR
	
Quick and accurate detection and quantification of target gene through real-time PCR