品牌:Active Motif

 

Data Sheet 下載

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基本資料:

 

  • Antibody Type: Polyclonal
  • Isotype: IgG
  • Purification: Protein A Chromatography
  • Host: Rabbit
  • Molecular Weight: 17 kDa
  • Reactivity: Budding Yeast, Human, Wide Range Predicted

 

應用:

  • ChIP
  • ChIP-Seq
  • WB
  • IF
  • IHC
  • ICC
  • Proximity Ligation Assay (PLA)

 

應用建議:

  • ChIP:5 µl per ChIP
  • ChIP-Seq:5 µl each
  • ICC/IF:1~5 µg/mL dilution
  • WB:0.1~1 µg/mL dilution

 

注意:為獲得最佳結果,我們建議在製備細胞核提取物時使用高鹽和超音波處理方式,實驗步驟下載

modENCODE validation:This antibody was validated for ChIP-Seq in this study (see references).

NGS-QC® certification:This antibody has been processed by the NGS-QC® generator.

 

抗原:

This Histone H3 acetyl Lys27 antibody was raised against a peptide including acetyl-lysine 27 of histone H3.

 

緩衝液:

Purified IgG in 10 mM sodium phosphate (pH 7.5), 150 mM NaCl, 30% glycerol, 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39135) of this antibody is also available.

 

Background:

Histone H3 is one of the core components of the nucleosome. The nucleosomeis the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine 27 of histone H3 can also be mono-, di- or trimethylated by different histone methyltransferases, such as EZH2 or NSD3. While histone methylationcan be associated with transcriptional activation or repression, methylation of Lysine 27 of histone H3 is mainly associated with transcriptional repression.

 

Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys27 antibody.

 

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K27ac antibody (pAb).

 

相容的二抗:

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K27ac antibody (pAb).

 

保存方式

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

 

保證

This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.

 

Application Key

ChIP = Chromatin Immunoprecipitation; DB = Dot Blot; ELISA = Enzyme-linked Immunosorbent Assay;EMSA = Electrophoretic Mobility Shift Assay; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA Immunoprecipitation; TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer; WB = Western Blotting

Histone H3K27ac antibody (pAb)

  • 貨號

    包裝

    AMF39133

    100 µg

    AMF39134

    10 µg

    AMF39034

    50 µg

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Fax:02-222-33-980
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