勝任細胞

SMB JM109 Competent Cells [JM109]

SMB試劑
特點
Rapid growing strain
Carries F' episome

介紹

A a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA.

Carries the F’ episome which allows blue/white screening for recombinant DNA.

A popular strain for routine subcloning.

Utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously.

Sensitive to all common antibiotics.

This product can become a powerful cloning tool when it is used in combination with ligase.

Grows fast, colonies to show up within 8 hours. Try this strain when you are in urgent.

 

Genotype

F' traD36 proA +  proB +  lacIq Δ(lacZ)M15 Δ(lac-proAB) supE44 hsdR17 recA1 gyrA96 thi¯1 endA1 relA1 e14¯ λ¯
 

應用:

  • Preparation of ssDNA
  • Construction of gene library
  • Subcloning

規格與訂購資訊

貨號
內容物 包裝
SMBE708
SMB JM109, >108 cfu/ug

100 μL/vial, 80 vials

Control Plasmid (pUC19): 5 μL (10-4 μg/μL)
SMBE709
SMB JM109, >5 x 108 cfu/ug

100 μL/vial, 80 vials

Control Plasmid (pUC19): 5 μL (10-4 μg/μL)
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