DNA-binding ELISA kits for studying activity of NFkB family members p50, p52, p65, c-Rel, and RelB

品牌:Active Motif



TransAM® NFκB Family (37篇)

TransAM® NFκB p50 (11篇)

TransAM® NFκB p52 (3篇)

TransAM® NFκB p65 (113篇)



NFκB Introduction & Background

Nuclear Factor κB (NFκB) is an important transcription factor complex that plays critical roles in regulating the expression of many genes in cells, including pathways that control the immune response, inflammation, development, cellular growth, and apoptosis. When NFκB is misregulated it can lead to many different disease states, including multiple types of cancer, neurodegenerative diseases, heart diseases, and inflammation. These disorders are usually due to overactive NFκB complexes, which highlights the importance of being able quickly, accurately, and reliably measure its activity in samples.

Read more about the NFκB transcription factor complex


Using TransAM DNA-Binding ELISAs to Measure NFκB Activity

TransAM NFκB kits are DNA-binding ELISAs that provide everything needed to study NFκB in biological samples, including a positive control extract. These assays are available with antibodies specific for the activated form of p50, p52, and p65. A family kit is also available with the ability to screen the members of the NFκB family (p50, p52, p65, c-Rel, and RelB).


Recombinant p50 and Recombinant p65 proteins are available separately to generate an optional protein standard curve in the TransAM NFκB Transcription Factor ELISA kits.


TransAM NF-kB Family Kit Contents & Storage

Kit components: One, two or five NFκB 96-well assay plate(s) with plate sealer(s), NFκB p50, p52 or p65 or p50, p52, p65, c-Rel and RelB primary antibody(ies), HRP-conjugated secondary antibody, NFκB wild-type and mutated oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Herring Sperm DNA, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing & Stop Solutions.

Kit storage: Reagent storage conditions vary from room temperature to -80°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.

NOTE: the p50 and p52 antibodies can be used with human and mouse extracts, while the p65 and RelB antibodies can be used with human, mouse, and rat extracts. The c-Rel antibody can only be used with human extracts.

Technology covered under EAT-filed patents and licensed to Active Motif. Use of TransAM in NFκB-related drug discovery may be covered under U.S. Patent No. 6,150,090 and require a license from Ariad Pharmaceuticals (Cambridge, MA, USA).



  • ELISA assay




TransAM® NFκB Activation Assays | Colorimetric Kits

  • 貨號




    TransAM® NFκB Family

    2 x 96 rxns


    TransAM® NFκB p50

    1 x 96 rxns


    TransAM® NFκB p50

    5 x 96 rxns


    TransAM® NFκB p52

    1 x 96 rxns


    TransAM® NFκB p52

    5 x 96 rxns


    TransAM® NFκB p65

    1 x 96 rxns


    TransAM® NFκB p65

    5 x 96 rxns