DNA-binding ELISA for activated HIF-1 transcription factor
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Assays are available for over 40 different targets (see the list at right). Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.
TransAM HIF-1 Kits provide everything needed to study activated Hypoxia Inducible Factor 1 alpha (HIF-1 alpha), including a positive control extract. The HIF-1 kit detects HIF-1 alpha in human and monkey samples.
- ELISA assay
1 x 96 rxns
5 x 96 rxns