Real-Time PCR (qPCR) Premix

SMB Real-time RT-PCR Kit

SMB試劑
特點
Ready-to-use master mix to allow faster setup, maximize throughput and reduce the contamination risk of real-time RT-PCR analysis
Detection of even low copy numbers
Excellent sensitivity and easy optimization with SYBR Green
Higher PCR efficiency and multiplexing

介紹

SMB Real-time SYBR Green RT-PCR system provides user with a rapid and simple way to quantify the expression of gene of interest based on Real-time PCR system containing SYBR Green. SMB Hipoint RT, HotStart DNA polymerase and all the components for Real-time SYBR Green RT-PCR are skillfully mixed within a single tube. Unique buffer system allows highly specific quantification by preventing the formation of nonspecific products and primer-dimers.

TaqMan detection system uses a designed fluorogenic probe to detect target PCR products during real-time PCR cycle. Fluorescent is detected when the quencher of probe is removed during the PCR extension cycle. SMB Real-time TaqMan RT-PCR kit complete reverse transcription and TaqMan Real-time PCR in a single tube. SMB Hipoint RT, HotStart DNA polymerase and all the components necessary for performing TaqMan Real-time PCR from RNA template are specially prepared to ensure high specificity and high sensitivity.
 

應用:

  • Validation of siRNA-mediated gene knockdown 
  • Detection of multiple gene expressions

規格與訂購資訊

型號
內容物 包裝
SMBT504-50P
SMB Real-Time SYBR Green RT PCR Kit 62.5 μL (50 reactions)
SMBT504-100P
SMB Real-Time SYBR Green RT PCR Kit 2 × 62.5 μL (100 reactions)
SMBT505-50P
SMB Real-Time TaqMan RT PCR Kit 62.5 μL (50 reactions)
SMBT505-100P
SMB Real-Time TaqMan RT PCR Kit 2 × 62.5 μL (100 reactions)
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