SMB GM2163 Competent Cells [GM2163]
產品說明:
An E. coli K12 strain, deficient in both dam and dcm genes thus is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes. 
Not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.
 
Genotype
F¯ ara¯14 leuB6 thi¯1 fhuA31 lacY1 tsx¯78 galK2 galT22 supE44 rpsL136(strr) xyl¯5 mtl¯1 dam13:Tn9 (camr) dcm¯6 mcrB1 hsdR2(rk¯mk + ) mcrA
 
 
特色:
    	
  • Chloramphenicol resistant
    • 	
  • Not suitable for blue/white screening
    • 	
  • Not recommended as a host for primary cloning/ligation
 
應用:
    	
  • Propagation of plasmid free of Dam and Dcm methylations
    • 	
  • Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes

SMB GM2163 Competent Cells [GM2163]