An E. coli K12 strain, deficient in both dam and dcm genes thus is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes.
Not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.
F¯ ara¯14 leuB6 thi¯1 fhuA31 lacY1 tsx¯78 galK2 galT22 supE44 rpsL136(strr) xyl¯5 mtl¯1 dam13:Tn9 (camr) dcm¯6 mcrB1 hsdR2(rk¯mk + ) mcrA
- Chloramphenicol resistant
- Not suitable for blue/white screening
- Not recommended as a host for primary cloning/ligation
- Propagation of plasmid free of Dam and Dcm methylations
- Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes
SMB GM2163 Competent Cells [GM2163]
貨號 品名 包裝 SMBE807 SMB GM2163, >1 x 107 cfu/ug
100 μL/vial, 80 vialsControl Plasmid (pUC19): 5 μL (10-4 μg/μL)