產品說明:

An E. coli K12 strain, deficient in both dam and dcm genes thus is suitable for the propagation of plasmid or cloned DNA to be cut with Dam or Dcm-sensitive restriction enzymes.

Not recommended as a host for primary cloning/ligation since the dam mutation can result in higher mutation rates and a reduction in the transformation efficiency.

 

Genotype

F¯ ara¯14 leuB6 thi¯1 fhuA31 lacY1 tsx¯78 galK2 galT22 supE44 rpsL136(strr) xyl¯5 mtl¯1 dam13:Tn9 (camr) dcm¯6 mcrB1 hsdR2(rk¯mk + ) mcrA

 

 

特色:

  • Chloramphenicol resistant
  • Not suitable for blue/white screening
  • Not recommended as a host for primary cloning/ligation

 

應用:

  • Propagation of plasmid free of Dam and Dcm methylations
  • Propagation of plasmid to be cut with Dam or Dcm-sensitive restriction enzymes

SMB GM2163 Competent Cells [GM2163]

  • 貨號 品名 包裝
    SMBE807 SMB GM2163, >1 x 107 cfu/ug

    100 μL/vial, 80 vials

    Control Plasmid (pUC19): 5 μL (10-4 μg/μL)

     

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