勝任細胞

SMB DH10β Competent Cells [DH10β]

SMB試劑
特點
Streptomycin resistant
Prevent DNA methylation
High transformation efficiency

介紹

An MC1061 derivative.

Designed for the propagation of large insert DNA library clones.

It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.

While DH10b has been classically reported to be galU galK, the preliminary genome sequence for DH10b indicates that DH10b is actually galE galK galU+. Genome sequence indicates that DH10b is actually deoR+.

Complete genome sequence has been published.

 

Genotype

F¯ endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80d lacZ ΔM15 araD139 Δ(lara,leu)7697 mcrA Δ(mrr¯hsdRMS¯mcrBC) λ¯

應用:

  • Suitable for genomic DNA cloning
  • Allow large inserts
  • Cloning of methylated cytosine or adenine containing DNA
  • Blue/white screening

規格與訂購資訊

貨號
內容物 包裝
SMBE508
SMB DH10b, >2 x 108 cfu/ug

100 μL/vial, 80 vials

Control Plasmid (pUC19): 5 μL (10-4 μg/μL)
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