產品說明:

An MC1061 derivative.

Designed for the propagation of large insert DNA library clones.

It is used extensively, taking advantage of properties such as high DNA transformation efficiency and maintenance of large plasmids.

While DH10b has been classically reported to be galU galK, the preliminary genome sequence for DH10b indicates that DH10b is actually galE galK galU+. Genome sequence indicates that DH10b is actually deoR+.

Complete genome sequence has been published.

 

Genotype

F¯ endA1 recA1 galE15 galK16 nupG rpsL ΔlacX74 Φ80d lacZ ΔM15 araD139 Δ(lara,leu)7697 mcrA Δ(mrr¯hsdRMS¯mcrBC) λ¯

 

 

特色:

  • Streptomycin resistant
  • Prevent DNA methylation
  • High transformation efficiency

 

 

應用:

  • Suitable for genomic DNA cloning
  • Allow large inserts
  • Cloning of methylated cytosine or adenine containing DNA
  • Blue/white screening

SMB DH10β Competent Cells [DH10β]

  • 貨號 品名 包裝
    SMBE508 SMB DH10b, >2 x 108 cfu/ug

    100 μL/vial, 80 vials

    Control Plasmid (pUC19): 5 μL (10-4 μg/μL)

     

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