SMB Accu DNA Polymerase
產品說明:
DNA polymerase derived from Pyrococcus Sp..
Catalyzes the incorporation of nucleotides into dsDNA in the 5'→3' direction in the presence of Mg2+ at 70 ~ 80℃.
Exhibits 3'→5' exonuclease (proofreading) activity, but has no detectable 5'→3' exonuclease activity.
Exhibits deoxynucleotidyl transferase activity, results in the addition of extra adenines at the 3’-end of PCR products, required for TA cloning
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
dUTP and dITP inhibit Accu DNA polymerase activity. Not recommended to use in PCR reaction with Accu.
3 different packages (including ready to use premix version, convenient and economic): see the order information
Produced by E. coli
 
 
特色:
    	
  • Yield: 1 kb/2min
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  • Highly thermostable: remains 95% activity after 2 hours incubation at 95℃
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  • Eight times more accurate than Taq DNA polymerase
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  • Error rate: 2.6 × 10-6 / nucleotide / cycle
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  • Generates blunt-end PCR products
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  • Amplicons: up to 3kb
 
應用:
    	
  • Cloning
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  • Amplification
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  • Mutation analysis
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  • Accurate polymerization
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  • Routine PCR amplification of DNA fragments up to 3 kb
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  • RT-PCR
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  • Blunt end amplification products

SMB Accu DNA Polymerase

  • 貨號 品名 包裝
    SMBT004-500U SMB Accu DNA Polymerase (w/ dNTPs) 100 μL (5 U/μl)*
    SMBT044-500U SMB Accu DNA Polymerase (w/o dNTPs) 100 μL (5 U/μl)*
    SMBT401-100P Accu DNA Polymerase Premix (2×) with Green dye 2 × 1.25 mL (100 reactions)

    *Unit Definition: The amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72℃.