產品說明:

DNA polymerase derived from Pyrococcus Sp..

Catalyzes the incorporation of nucleotides into dsDNA in the 5'→3' direction in the presence of Mg2+ at 70 ~ 80.

Exhibits 3'→5' exonuclease (proofreading) activity, but has no detectable 5'→3' exonuclease activity.

Exhibits deoxynucleotidyl transferase activity, results in the addition of extra adenines at the 3’-end of PCR products, required for TA cloning

Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).

dUTP and dITP inhibit Accu DNA polymerase activity. Not recommended to use in PCR reaction with Accu.

3 different packages (including ready to use premix version, convenient and economic): see the order information

Produced by E. coli

 

 

特色:

  • Yield: 1 kb/2min
  • Highly thermostable: remains 95% activity after 2 hours incubation at 95
  • Eight times more accurate than Taq DNA polymerase
  • Error rate: 2.6 × 10-6 / nucleotide / cycle
  • Generates blunt-end PCR products
  • Amplicons: up to 3kb

 

應用:

  • Cloning
  • Amplification
  • Mutation analysis
  • Accurate polymerization
  • Routine PCR amplification of DNA fragments up to 3 kb
  • RT-PCR
  • Blunt end amplification products

SMB Accu DNA Polymerase

  • 貨號 品名 包裝
    SMBT004-500U SMB Accu DNA Polymerase (w/ dNTPs) 100 μL (5 U/μl)*
    SMBT044-500U SMB Accu DNA Polymerase (w/o dNTPs) 100 μL (5 U/μl)*
    SMBT401-100P Accu DNA Polymerase Premix (2×) with Green dye 2 × 1.25 mL (100 reactions)

    *Unit Definition: The amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72.

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