產品說明:
DNA polymerase derived from Pyrococcus Sp..
Catalyzes the incorporation of nucleotides into dsDNA in the 5'→3' direction in the presence of Mg2+ at 70 ~ 80℃.
Exhibits 3'→5' exonuclease (proofreading) activity, but has no detectable 5'→3' exonuclease activity.
Exhibits deoxynucleotidyl transferase activity, results in the addition of extra adenines at the 3’-end of PCR products, required for TA cloning
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).
dUTP and dITP inhibit Accu DNA polymerase activity. Not recommended to use in PCR reaction with Accu.
3 different packages (including ready to use premix version, convenient and economic): see the order information
Produced by E. coli
特色:
- Yield: 1 kb/2min
- Highly thermostable: remains 95% activity after 2 hours incubation at 95℃
- Eight times more accurate than Taq DNA polymerase
- Error rate: 2.6 × 10-6 / nucleotide / cycle
- Generates blunt-end PCR products
- Amplicons: up to 3kb
應用:
- Cloning
- Amplification
- Mutation analysis
- Accurate polymerization
- Routine PCR amplification of DNA fragments up to 3 kb
- RT-PCR
- Blunt end amplification products
SMB Accu DNA Polymerase
貨號 品名 包裝 SMBT004-500U SMB Accu DNA Polymerase (w/ dNTPs) 100 μL (5 U/μl)* SMBT044-500U SMB Accu DNA Polymerase (w/o dNTPs) 100 μL (5 U/μl)* SMBT401-100P Accu DNA Polymerase Premix (2×) with Green dye 2 × 1.25 mL (100 reactions) *Unit Definition: The amount of enzyme that will incorporate 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 72℃.