- Antibody Type: Polyclonal
- Isotype: Serum
- Purification: None
- Host: Rabbit
- Molecular Weight: 17 kDa
- Reactivity: Fission Yeast, Human, Wide Range Predicted
- ChIP: 2 ~ 5 µl per ChIP
- ChIP-Seq: 5 µl each
- ICC/IF: 1:500 ~ 1:1,000 dilution
- WB: 1:1,000 ~ 1:5,000 dilution
This Histone H3 trimethyl Lys9 antibody was raised against a peptide including trimethyl-lysine 9 of histone H3.
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39765) of this antibody that was purified by Protein A Chromatography is also available.
Histone H3 is one of the core components of the nucleosome. The nucleosomeis the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.
The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 9 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SuvH39H1 or G9a. This methylated lysine can be demethylated by histone demethylases as JMJD1A, LSD1 or JMJD2C. Methylation of this residue is mainly associated with transcriptional repression.
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 trimethyl Lys9 antibody.
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K9me3 antibody (pAb).
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K9me3 antibody (pAb).
Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
ChIP = Chromatin Immunoprecipitation; DB = Dot Blot; ELISA = Enzyme-linked Immunosorbent Assay;EMSA = Electrophoretic Mobility Shift Assay; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA Immunoprecipitation; TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer; WB = Western Blotting
Histone H3K9me3 antibody (pAb)