品牌：Active Motif
 
Data Sheet 下載
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基本資料：
	
	
Antibody Type: Polyclonal
	
Isotype: Serum
	
Purification: None
	
Host: Rabbit
	
Molecular Weight: 17 kDa
	
Reactivity: Budding Yeast, Human, Mouse, Wide Range Predicted
	
 
應用：
	
ChIP
	
ChIP-Seq
	
WB
	
IF
	
IHC
	
ICC
	
FC 
	
Proximity Ligation Assay (PLA)
 
應用建議：
	
ChIP：3~5 µl per ChIP
	
ChIP-Seq：3 µl each
	
ICC/IF：1:500 ~ 1:1,000 dilution
	
WB：1:500 ~ 1:2,000 µg/mL dilution
 
注意：modENCODE validation. This antibody was validated for ChIP-Seq in this study (see reference).NGS-QC® certification. This antibody has been processed by the NGS-QC® generator. For additional details, click here.
 
抗原：
This Histone H3 trimethyl Lys4 antibody was raised against a peptide including trimethyl-lysine 4 of histone H3.
 
緩衝液：
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 39915) of this antibody that was purified by Protein A Chromatography is also available.
 
Background：
Histone H3 is one of the core components of the nucleosome. The nucleosomeis the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points. Histone H1 is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 4 of histone H3 can be mono-, di- or trimethylated by different histone methyltransferases (HMTs) such as SET1 or ASH1. Methylation of Lys4 is often associated with transcriptional activation. The demethylase LSD1 is able to demethylate histone H3 Lys4.
 
Positive Control
Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 trimethyl Lys4 antibody.
 
qPCR Primers
Active Motif's Human Positive Control Primer Set ACTB-2 (Cat. No. 71005) and Human Negative Control Primer Set 1 (Cat. No. 71001) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Actb-2 (Cat. No. 71017) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.
 
Chromatin IP Assays
This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K4me3 antibody (pAb).
 
相容的二抗：
Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K4me3 antibody (pAb).
 
保存方式
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
 
保證
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
 
Application Key
ChIP = Chromatin Immunoprecipitation; DB = Dot Blot; ELISA = Enzyme-linked Immunosorbent Assay;EMSA = Electrophoretic Mobility Shift Assay; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA Immunoprecipitation; TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer; WB = Western Blotting