品牌:Active Motif

 

Data Sheet 下載

文獻清單

 

基本資料:

  • Antibody Type: Polyclonal
  • Isotype: IgG
  • Purification: Protein A Chromatography
  • Host: Rabbit
  • Molecular Weight: 17 kDa
  • Reactivity: Human, Mouse, Wide Range Predicted

 

應用:

  • ChIP
  • ChIP-Seq
  • WB
  • IF
  • IHC
  • ICC
  • Proximity Ligation Assay (PLA)

 

應用建議:

  • ChIP:5~10 µl per ChIP
  • ChIP-Seq:5 µl each
  • ICC/IF:2 µg/mL dilution
  • IHC (FFPE): 2 µg/ml dilution
  • WB:0.5~2 µg/mL dilution

 

注意:為獲得最佳結果,我們建議在製備細胞核提取物時使用高鹽和超音波處理方式,實驗步驟下載

The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H3 trimethyl Lys27 antibody was validated for ChIP-Seq in the study (see reference).

 

抗原:

This Histone H3 trimethyl Lys27 antibody was raised against a peptide including trimethyl-lysine 27 of histone H3. testing

 

緩衝液:

Purified IgG in PBS (pH 7.5) with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an unpurified serum version (Catalog No. 39156) of this antibody is also available.

 

Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for Histone H3 trimethyl Lys27 antibody.

 

qPCR Primers

Active Motif's Human Positive Control Primer Set MYT1 (Cat. No. 71007) and Human Negative Control Primer Set 3 (Cat. No. 71023) have been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Pax-2 (Cat. No. 71020) and Mouse Negative Control Primer Set 1 (Cat. No. 71011) have been validated for both qPCR and endpoint PCR.

 

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3K27me3 antibody (pAb).

 

相容的二抗:

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H3K27me3 antibody (pAb).

 

保存方式

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

 

保證

This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.

 

Application Key

ChIP = Chromatin Immunoprecipitation; DB = Dot Blot; ELISA = Enzyme-linked Immunosorbent Assay;EMSA = Electrophoretic Mobility Shift Assay; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA Immunoprecipitation; TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer; WB = Western Blotting

Histone H3K27me3 antibody (pAb)

  • 貨號

    包裝

    AMF39155

    100 µg

    AMF39157

    10 µg

    AMF39055

    50 µg

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