品牌:Active Motif

 

Data Sheet 下載

文獻清單

 

基本資料:

  • Antibody Type: Polyclonal
  • Isotype: Serum
  • Purification: None
  • Host: Rabbit
  • Molecular Weight: 17 kDa
  • Reactivity: Human, Wide Range Predicted

 

應用:

  • ChIP, ChIP-Seq
  • WB
  • DB

 

應用建議:

  • ChIP:10 µl per ChIP
  • ChIP-Seq:5 µl each
  • WB:1:500 ~ 1:5,000 dilution

 

注意:為獲得最佳結果,我們建議在製備細胞核提取物時使用高鹽和超音波處理方式,實驗步驟下載

 

 

抗原:

This Histone H3 acetyl antibody was raised against a peptide including acetyl-lysines contained in the N-terminal tail of histone H3.

 

緩衝液:

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic. For your convenience, an IgG version (Catalog No. 61637) of this antibody that was purified by Protein A Chromatography is also available.

 

Background:

Histone H3 is one of the core components of the nucleosome. The nucleosomeis the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Acetylation of histone H3 occurs at several different lysine positions in the histone tail, and is performed by Histone Acetyltransferases (HATs) such as CBP/p300. Acetylation of histones is often associated with transcriptional activation.

 

Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H3 acetyl Lys27 antibody.

 

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H3ac (pan-acetyl) antibody (pAb).

 

保存方式

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

 

保證

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Application Key

ChIP = Chromatin Immunoprecipitation; DB = Dot Blot; ELISA = Enzyme-linked Immunosorbent Assay;EMSA = Electrophoretic Mobility Shift Assay; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA Immunoprecipitation; TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer; WB = Western Blotting

Histone H3ac (pan-acetyl) antibody (pAb)

  • 貨號

    包裝

    AMF39139

    100 µg

    AMF39140

    10 µg

    AMF39040

    50 µg

Tel:02-222-33-900
Fax:02-222-33-980
23586新北市中和區中正路928號4樓
4F., No.928, Zhongzheng Rd., Zhonghe Dist., New Taipei City 235, Taiwan (R.O.C.)
Email:smt@star-moon.com.tw
© 2016 by STARMOONTECH.