to quantitate the level of histone H3 lysine 9 methylation
Histone Modification ELISAs provide a simple, sensitive method for detecting changes in histone methylation levels from purified core histones or histones isolated by acid extraction. For added convenience and a more quantitative interpretation of results, the Histone Modification ELISAs also include a site- and degree-specific methylated recombinant histone protein for use as a reference standard curve.
Histone H3 lysine 9 methylation (H3K9) has been correlated with heterochromatin formation and transcriptional repression, making the methylation state of lysine 9 an interesting marker of transcriptional activity. Histone H3 methylated Lys9 ELISAs are sandwich ELISAs for screeing the levels of histone H3 dimethyl or trimethyl Lys9 in your samples. The methylation specific histone H3 antibodies will not cross-react with other site- or degree-specific methylated histones, enabling small changes in methylation levels to be detected. Click on the H3K9 Info tab below for data and more information.
- ELISA assay
Histone H3 methylated Lys9 ELISA (H3K9)
Histone H3 trimethyl Lys9 ELISA
1 x 96 rxns