to quantitate the level of histone H3 lysine 27 methylation
Histone Modification ELISAs provide a simple, sensitive method for detecting changes in specific histone modifications from purified core histones or histones isolated by acid extraction. These kits are sandwich ELISAs that utilize a capture antibody against histone H3 and a primary antibody specifc for the modification of interest. A secondary antibody conjugated to horseradish peroxidase (HRP) and developing solutions provide a sensitive colorimetric readout that is easily quantified by spectrophotometry. The assay is performed in a convenient 96-stripwell plate, enabling low or high throughput screening.
Histone H3 lysine 27 methylation ELISA kits provide everything needed for screening the levels of histone H3 mono- or trimethyl Lys 27 in human, mouse, rat and other (wide range predicted) systems. Histone H3 lysine 27 methylation (H3K27) has been correlated with heterochromatin formation and transcriptional repression, making the methylation state of lysine 27 an interesting marker of transcriptional activity. The methylation specific histone H3 antibodies will not cross-react with other site- or degree-specific methylated histones, enabling small changes in methylation levels to be detected. For added convenience and a more quantitative interpretation of results, the Histone Modification ELISAs also include a site- and degree-specific methylated recombinant histone protein for use as a reference standard curve.
- ELISA assay
Histone H3 methylated Lys27 ELISA (H3K27)
Histone H3 monomethyl Lys27 ELISA
1 x 96 rxns
Histone H3 trimethyl Lys27 ELISA
1 x 96 rxns