to quantitate the level of histone H3 lysine 27 methylation


品牌:Active Motif



Histone Modification ELISAs provide a simple, sensitive method for detecting changes in specific histone modifications from purified core histones or histones isolated by acid extraction. These kits are sandwich ELISAs that utilize a capture antibody against histone H3 and a primary antibody specifc for the modification of interest. A secondary antibody conjugated to horseradish peroxidase (HRP) and developing solutions provide a sensitive colorimetric readout that is easily quantified by spectrophotometry. The assay is performed in a convenient 96-stripwell plate, enabling low or high throughput screening.


Histone H3 lysine 27 methylation ELISA kits provide everything needed for screening the levels of histone H3 mono- or trimethyl Lys 27 in human, mouse, rat and other (wide range predicted) systems. Histone H3 lysine 27 methylation (H3K27) has been correlated with heterochromatin formation and tran