品牌:Active Motif

 

Data Sheet 下載

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基本資料:

  • Antibody Type: Polyclonal
  • Isotype: IgG
  • Purification: Affinity Purified
  • Host: Rabbit
  • Molecular Weight: 120kDa
  • Reactivity: Human

 

應用:

  • ChIP
  • ChIP-Seq
  • WB
  • IF
  • IHC
  • EMSA
  • ICC

 

應用建議:

  • ChIP: 2 - 8 µl per ChIP
  • ChIP-Seq: 4 µg per ChIP
  • ICC/IF: 1:2,000 dilution
  • WB: 1:500 ~ 1:2,000 dilution
  • IHC (FFPE): 1:1000 dilution

 

注意:For optimal results, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts. /visit activemotif.com to download the protocol.

 

抗原:

This CTCF antibody was raised against a peptide within the N-terminal region of human CTCF.

 

緩衝液:

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Background:

CTCF (CCCTC-binding factor, zinc finger protein) is a chromatin binding factor that binds to DNA sequence specific sites. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Acts as transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene. Also binds to the PLK and PIM1 promoters. Acts as a transcriptional activator of APP. Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression. Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription. Seems to act as tumor suppressor. Plays a critical role in the epigenetic regulation. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2. Plays a critical role in gene silencing over considerable distances in the genome. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones. Inversely, binding to target sites is prevented by CpG methylation. Plays a important role in chromatin remodeling. Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping. Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory. Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription. When bound to chromatin, it provides an anchor point for nucleosomes positioning. Seems to be essential for homologous X-chromosome pairing. May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation. May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites. Regulates asynchronous replication of IGF2/H19.

 

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for CTCF antibody.

 

qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Negative Control Primer Set 1 (Cat. No. 71011) has been validated for both qPCR and endpoint PCR.

 

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with CTCF antibody (pAb).

 

相容的二抗:

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CTCF antibody (pAb).

 

保存方式

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

 

保證

This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.

 

Application Key

ChIP = Chromatin Immunoprecipitation; DB = Dot Blot; ELISA = Enzyme-linked Immunosorbent Assay;EMSA = Electrophoretic Mobility Shift Assay; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA Immunoprecipitation; TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer; WB = Western Blotting

CTCF antibody (pAb)

  • 貨號

    包裝

    AMF39097

    200 µg

    AMF39497

    50 µg

Tel:02-222-33-900
Fax:02-222-33-980
23586新北市中和區中正路928號4樓
4F., No.928, Zhongzheng Rd., Zhonghe Dist., New Taipei City 235, Taiwan (R.O.C.)
Email:smt@star-moon.com.tw
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