品牌:Active Motif

 

Data Sheet 下載

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基本資料:

  • Antibody Type: Polyclonal
  • Isotype: Serum
  • Purification: None
  • Host: Rabbit
  • Reactivity: Human, Mouse, Not Species Specific

 

應用:

  • MeDIP
  • MeDIP-Seq
  • IF
  • DB
  • FC
  • IHC
  • ICC

 

應用建議:

  • MeDIP: 0.1 ~ 0.5 µL per IP
  • DB: 1:10,000 dilution

 

注意:為獲得最佳結果,我們建議在製備細胞核提取物時使用高鹽和超音波處理方式,實驗步驟下載

modENCODE validation:This antibody was validated for ChIP-Seq in this study (see references).

NGS-QC® certification:This antibody has been processed by the NGS-QC® generator.

 

抗原:

This 5-Hydroxymethylcytosine antibody was raised against 5-hydroxymethylcytidine conjugated to KLH and recognizes 5-hydroxymethylcytosine.

 

緩衝液:

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Background:

Active Motif offers two polyclonal antibodies that recognize 5-hydroxymethylcytosine, a whole serum version (39769) and a purified IgG version (39791). Both are validated for use in methyl DNA immunoprecipitation (MeDIP). For customers who must quantitate the amount of IgG in the MeDIP reaction, the purified IgG version (39791) is recommended. The whole serum version (39769) is very high titre, so should be used carefully (0.1 - 0.5 µl per IP) to prevent non-specific background. The whole serum version (39769) has been used successfully in immunofluorescence (IF, Ito et al, 2010);the purified IgG version (39791) is likely to work in IF as well.

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates.

A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. This antibody was developed specifically to distinguish 5-hydroxymethylcytosine from 5-methylcytosine as conventional methods (enrichment by antibody or methyl DNA binding protein, enzymatic digestion and bisulfite sequencing) cannot do so. It is possible that 5-hydroxymethylcytosine(5-hmC) represents a pathway to demethylate DNA, as 5-hydroxymethylcytosine is repaired as mismatched DNA and replaced with unmethylated cytosine.

 

相容的二抗:

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with 5-Hydroxymethylcytosine (5-hmC) antibody (pAb).

 

保存方式

Antibodies in solution can be stored at -20°C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

 

保證

This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.

 

Application Key

ChIP = Chromatin Immunoprecipitation; DB = Dot Blot; ELISA = Enzyme-linked Immunosorbent Assay;EMSA = Electrophoretic Mobility Shift Assay; FC = Flow Cytometry; ICC = Immunocytochemistry; IF = Immunofluorescence; IHC = Immunohistochemistry; IP = Immunoprecipitation; MeDIP = Methyl-DNA Immunoprecipitation; TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer; WB = Western Blotting

5-Hydroxymethylcytosine (5-hmC) antibody (pAb)

  • 貨號

    包裝

    AMF39769

    100 µg

    AMF39770

    10 µg

    AMF39069

    50 µg

Tel:02-222-33-900
Fax:02-222-33-980
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4F., No.928, Zhongzheng Rd., Zhonghe Dist., New Taipei City 235, Taiwan (R.O.C.)
Email:smt@star-moon.com.tw
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